Studies on panicle characters and yield in relation to panicle numbers in rice cultivars

報告番号: 甲26337 ; 学位授与年月日: 2010-09-07 ; 学位の種別: 課程博士 ; 学位の種類: 博士(農学) ; 学位記番号: 博農第3611号 ; 研究科・専攻: 農学生命科学研究科生産・環境生物学専

Solvent Exchange in a Hele-Shaw Cell Universality of Surface Nanodroplet Nucleation

Solvent exchange (also called solvent shifting or Ouzo effect) is a generally used bottom-up process to mass-produce nanoscale droplets. In this process, a good solvent for some oil is displaced by a poor one, leading to oil nanodroplet nucleation and subsequent growth. Here we perform this process on a hydrophobic substrate so that sessile droplets so-called surface nanodroplets-develop, following the work of Zhang et al. [Zhang, X.; Lu, Z.; Tan, H.; Bao, L.; He, Y.; Sun, C.; Lohse, D. Proc. Natl. Acad. Sci. U.S.A. 2015, 122, 9253-9257]. In contrast to what was done in that paper, we chose a very well-controlled Hele-Shaw geometry with negligible gravitational effects, injecting the poor solvent in the center of the Hele-Shaw cell, and characterize the emerging nanodroplets as a function of radial distance and flow rates. We find that the mean droplet volume per area _area strongly depends on the local Peclet number Pe and follows a universal scaling law _area~Pe^(3/4). Moreover, the probability distribution function of the droplet volume strongly depends on the local Pe as well, regardless of the flow rates and radial distance, giving strong support to the theoretical model of the solvent exchange process developed in Zhang et al.'s work.Comment: 6 pages, 5 figure

Quantitative evaluation of soil ion content using an imaginary part model of soil dielectric constant

An imaginary part model of soil dielectric constant for predicting the soil salinity status was developed based on a series of relations between dielectric imaginary part and soil bulk conductivity, soil bulk conductivity and soil solution electrical conductivity, and soil solution electrical conductivity and ion contents in soil using pot trials with different soil salinity levels in the 2008 growing season. This model was calibrated and tested with data from the 2009 growing season. The results showed that the inverted values of the total concentration of salt (Sc), Cl– , and Ca2+ at low frequencies (P-band of microwave observations) from the imaginary part model fitted well with the observed values, since root mean square errors (RMSEs) were 0.34 g kg–1, 0.09 g kg–1 and 0.13 g kg–1, respectively, but the inversion effect of Na+ was relatively poor. Moreover, the Sc, Cl– , and Na+ could be well inverted at high frequencies (C-band of microwave observations), since RMSEs were minor, with values of 0.25 g kg–1, 0.02 g kg–1, and 0.15 g kg–1, respectively. The close fit between the observed and inverted values indicated that the present models could be used to estimate soil ion content quickly and reliably under different saline conditions, which, when suitable measures are taken, can be used to reduce the effects of soil salinity on crop growth

When are multidegrees positive?

Let $k$ be an arbitrary field, $P = P_k^{m_1} \times_k \cdots \times_k P_k^{m_p}$ be a multiprojective space over $k$, and $X \subseteq P$ be a closed subscheme of $P$. We provide necessary and sufficient conditions for the positivity of the multidegrees of $X$. As a consequence of our methods, we show that when $X$ is irreducible, the support of multidegrees forms a discrete algebraic polymatroid. In algebraic terms, we characterize the positivity of the mixed multiplicities of a standard multigraded algebra over an Artinian local ring, and we apply this to the positivity of mixed multiplicities of ideals. Furthermore, we use our results to recover several results in the literature in the context of combinatorial algebraic geometry.Comment: This is a generalized and expanded version of arXiv:1612.00154. Final version, to appear in Advances in Mathematic

Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro: Implications for the role of NLS in viral replication

<p>Abstract</p> <p>Background</p> <p>Porcine circovirus type 2 (PCV2) is believed to be the primary causative agent of postweaning multisystemic wasting syndrome (PMWS). It is supposed that capsid protein of PCV may contribute to replication control via interaction between Cap and Rep in the nucleoplasm. In this study, we described the construction and in vitro characterization of NLS-exchanged PCV DNA clones based on a PMWS-associated PCV2b isolate from China to determine the role of ORF2 NLS in PCV replication.</p> <p>Results</p> <p>The PCV1, PCV2, PCV2-NLS1 and PCV1-NLS2 DNA clone were generated by ligating a copy of respective genome in tandem with a partial duplication. The PCV2-NLS1 and PCV1-NLS2 DNA clone contained a chimeric genome in which the ORF2 NLS was exchanged. The four DNA clones were all confirmed to be infectious in vitro when transfected into PK-15 cells, as PCV capsid protein were expressed in approximately 10-20% of the transfected cells. The in vitro growth characteristics of the DNA clones were then determined and compared. All the recovered progeny viruses gave rise to increasing infectious titers during passages and were genetically stable by genomic sequencing. The chimeric PCV1-NLS2 and PCV2-NLS1 viruses had the final titers of about 10^4.2and 10^3.8TCID₅₀/ml, which were significantly lower than that of PCV1 and PCV2 (10^5.6and 10^5.0TCID₅₀/ml, respectively). When the ORF2 NLS exchanged, the mutant PCV2 (PCV2-NLS1) still replicated less efficiently and showed lower infectious titer than did PCV1 mutant (PCV1-NLS2), which was consistent with the distinction between wild type PCV1 and PCV2.</p> <p>Conclusions</p> <p>Recovery of the chimeiric PCV1-NLS2 and PCV2-NLS1 progeny viruses indicate that the nuclear localization signal sequence of capsid protein are functionally exchangeable between PCV1 and PCV2 with respect to the role of nuclear importing and propagation. The findings also reveal that ORF2 NLS play an accessory role in the replication of PCV. However, we found that ORF2 NLS was not responsible for the distinction of in vitro growth characteristic between PCV1 and PCV2. Further studies are required to determine the in vivo viral replication and pathogenicity of the NLS chimeric DNA clones.</p